Abstract: To determinate the catechins content of tea sample, three catech in s catechin(C), epicatechin(EC) and epigallocatechin gallate (EGCG) were used as standard samples, respectively. As the result, the catechins purities of tea pol yphenol with different standard sample was 51.98%, 49.59% and 82.39%, the re lative standard (RSD) were 0.65%, 1.79% and 2.28%, the average recoveries we re 94.19%, 93.46% and 102.4%, respectively. To compared with the ultraviolet spectrophotometric method, HPLC was used to identify and determinate the tea c a techins and C, EC, EGC, ECG and EGCG were successfully separated. The result s ho wed that the purity of each was 5.05%, 10.38%, 25.96%, 1.98% and 10.18%, r espectively. The whole catechins purity of tea polyphenol sample was 53.5% w hi ch was in agreement with that of UV method (catechin was the standard sample).

Key words: catechin, UV, HPLC, determination