FOOD SCIENCE ›› 2003, Vol. 24 ›› Issue (4): 110-113.

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Monosaccharide Composition Analysis in Crude Polysaccharide and Polysaccharide Fractions from Grifola Frondosa by Gas Chromatography

 LI  Xiao-Ding, RONG  Jian-Hua, WU  Mou-Cheng   

  • Online:2003-04-15 Published:2011-12-13

Abstract: The separation and determination of monosacchride composition in cude polysaccharide and polysaccharide fractions from Grifola frondosa is reported by gas chromatography with OV-1701 capillary colume. Monosacchrides from polysacc harides hydrolyzed with 2.0mol/L CF3COOH are reduced and derivatized to sugar ac etylated aldononitriles before detemination. The linear correlation coefficients of 7 monosaccharides were all over 0.997, the recoveries of monosaccharides ran ged 98.5%~101.6%, and the relative standard deviations were below 2%.

Key words: Grifola frondosa, polysaccharide, gas chromatography, monosacchride