Enzymatic Kinetics Study on Polyphenol Oxidase of Highbush Blueberry

Abstract

Abstract: The substrate specificity of highbush blueberry polyphenol (Vaccyn iu m corybosum L.) oxidase has been studied by a substrate scanning method in spec trophotometric range. The enzymeatic kinetics has been investigated by using th e spectrophotometric method and poloragraphe method. Michaelis-constant(Km) has been assayed. The kinds of inhibitors and the effects of inhibitor concentrati o n and of combination time of inhibitor and enzyme on the inhibition of PPO hav e also been stadied.

Key words: PPO, substrate specificity, kinetics of enzyme, Michaelis-constant, inhib itor

YANG Meng, B.Rovel , M..Metche . Enzymatic Kinetics Study on Polyphenol Oxidase of Highbush Blueberry[J]. FOOD SCIENCE, 2003, 24(4): 55-58.

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Enzymatic Kinetics Study on Polyphenol Oxidase of Highbush Blueberry

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