Transcriptome Sequencing of Agaricus bisporus and Mining of Genes Involved in Browning

doi:10.7506/spkx1002-6630-20180510-154

Abstract

Abstract: In order to study how polyphenol oxidase regulates the progression of Agaricus bisporus browning, we performed mRNA sequencing using the RNA-seq technique on the pilei of A. bisporus collected on day 1, 7 and 14 of postharvest storage. A total of 168 607 212 high-quality reads were obtained; 727 and 1 524 differentially expressed genes were identified on day 7 and 14 compared with day 1 of storage, respectively. These differentially expressed genes were classified into 180 metabolic pathways after gene ontology and pathway enrichment analysis; of them, five polyphenol oxidase genes (PPO1, PPO3, PPO5, LAC1 and LAC4) were screened out and their relationships with browning were investigated. These results provide a theoretical basis for mining of functional genes and quality improvement of A. bisporus.

Key words: Agaricus bisporus, browning, RNA-seq, differentially expressed genes (DEGs), polyphenol oxidase (PPO)

CLC Number:

S646.1

PENG Bo, LI Bingjuan, GUAN Wenqiang, LIN Qiong. Transcriptome Sequencing of Agaricus bisporus and Mining of Genes Involved in Browning[J]. FOOD SCIENCE, 2019, 40(2): 126-132.

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Transcriptome Sequencing of Agaricus bisporus and Mining of Genes Involved in Browning

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