FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (11): 171-173.

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The Ligation of Gene xanA in Xanthomonas campestris and pUCm-T Vector

 XI  Ling, LI  Bai-Lin,   欧Jie   

  1. College of Food Science,Shanghai Fisheries University,Shanghai 200090,China
  • Online:2006-11-15 Published:2011-11-28

Abstract: Two methods for gene xanA to ligate with pUCm-T vector were discussed in this thesis. And the transformations were also done for testing. It showed that the better ligation condition was ligated at 16℃ for overnight. In the ligation system,the volume ratio of gene xanA to pUCm-T vector was 4:1.

Key words:  , Xanthomonas campestris; gene xanA; pUCm-T vector; ligation;