Cloning of Producing Colloid Gene GumD from Xanthomonas campestris and Constructing of Recombinant Plasmid

Abstract

Abstract: Objective: To establish the methods of cloning gene GumD from Xanthomonas campestris and constructing recombinant plasmid. MethodThe fragment of the producing colloid gene GumD from Xanthomonas campestris was amplified by the optimized experiment of PCR method,then the gene GumD and plasmid pMD18- T with drug-resistant qualities was connected. In order to clone and rebuild the recombinant plasmid,E. coli JM109 was transformed with the recombinant plasmid by way of CaCl2. ResultCompared with the sequence from the gene bank,the correct sequence of recombinant plasmid was confirmed by DNA sequencing. Conclusion: This approach could be used to clone producing colloid gene GumD and construct recombinant plasmid.

Key words: , Xanthomonas campestris； producing colloid gene； cloning； recombinant plasmid；

欧Jie , Li-Bai-Lin, Liu-Ying, Xu-Ming-Quan. Cloning of Producing Colloid Gene GumD from Xanthomonas campestris and Constructing of Recombinant Plasmid[J]. FOOD SCIENCE, 2006, 27(11): 182-184.

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Cloning of Producing Colloid Gene GumD from Xanthomonas campestris and Constructing of Recombinant Plasmid

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