Abstract: Curcuminoids from the alcohol extract of Curcuma Longa L root were readily separated by HPLC equipped with a PDA detector on a C30 column under following conditionStationary phase=YMCTM Carotenoid S-5 column (250×4.6mm); Mobile phase A=acetonitrile-water (80:20,V/V) with pH 2.5 adjusted by phosphate; Mobile phase B=acetonitrile:water (95: 5,V/V) with pH 2.5 adjusted by phosphate; Linear gradient: B increased from 0 to 100% (V/V) in 15 minutes; Flow rate=1.0ml/ min. PDA wavelength range: 300～550nm; Sample injection volume=20μl. According to their chromatographic behavior and spectral characters,three homologues,curcumin,demethoxy curcumin and bisdemethoxy curcumin,were identified. Results of this study would form a base to quantify curcuminoid homologues by C30-HPLC-PDA.

Key words:  , curcuminoids； C30 column； HPLC；