FOOD SCIENCE ›› 2003, Vol. 24 ›› Issue (1): 48-51.

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Study on Optimum Minitype Fermentation Conditions of Lipid Production by Rhodotorule Glurtinis GLR513

 SHI  An-Hui, ZHOU  Bo   

  • Online:2003-01-15 Published:2011-12-13

Abstract: Weobtaineda Rhodoruleglutinis mutantGLR 513 byprotoplasts mutagenization(UV.andEMS )tobeput into small scale lipid production .T he best minitype fermentation condi tions as to use glucose as carbon sour ce,(NH 4 ) 2 SO 4 as nitrogen source,C /Nas 70∶1,starting pHas 5.5,inoculative amount as20%and temperature as30℃.The yield of lipid production co uld reach 67.2%of dry thallius weigh t by replenishing carbon source in anaphase for 72h .We confirmed the co mponent of lipid by gas -liquid chrom atogrphy and mass spectrum: 2003,Vol.24,No.1palmitoleic acid 33.31%,oleic acid3.80%,γ-linoleic acid 0.20%,EPA2.60%and DHA3.60%with the amount as 56.61%.

Key words: Rhodotoruleglutinis, fermentation condition, componen t and constant of lipid