Optimization of Fermentation Conditions for Protease Production by Bacillus licheniformis with Enteromorpha prolifera as Carbon Source

doi:10.7506/spkx1002-6630-201607022

Abstract

Abstract:

This work reports on the selection and optimization of culture conditions and medium components as well as their
levels for improved production of protease by Bacillus licheniformis with Enteromorpha prolifera as a carbon source by the
combined use of single factor method, Plackett-Burman design and response surface methodology (RSM). Initial medium
pH, Enteromorpha prolifera concentration and NaH2PO4 concentration were identified as the most significant factors that
influence protease production. The levels of the three factors were optimized using RSM to be 6.66, 4.65% and 0.027 4%,
respectively. Under the optimized conditions, the predicted values of protease activity was 66 354.70 U/g protein and an
average value of 66 966.37 U/g protein was obtained from three replicate experiments, which was 3.68 times higher than that
before optimization.

Key words: Bacillus licheniformis, Enteromorpha prolifera, fermentation conditions, protease, Plackett-Burman design

CLC Number:

Q939.9

WANG Ying, GAO Xiang, ZHOU Jun, ZHANG Chundan, LI Ye, WANG Zuzhong, YUAN Bei, DAI Juan, QIAN Qinlian, SU Xiurong. Optimization of Fermentation Conditions for Protease Production by Bacillus licheniformis with Enteromorpha prolifera as Carbon Source[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201607022.

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Optimization of Fermentation Conditions for Protease Production by Bacillus licheniformis with Enteromorpha prolifera as Carbon Source

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