FOOD SCIENCE ›› 2000, Vol. 21 ›› Issue (1): 31-33.

Previous Articles     Next Articles

 XIAO  Li-Xia, SHI  Yong-Chang, JIANG  Yong-Ming   

  • Online:2000-01-15 Published:2011-11-25

Abstract: A crude thrombin (ECe.4.21.5)was obtained from porcine plasma by means of precipitation at isoeletric point. The thrombin was purified by affinity chromatography on the heparin which was immobilized on chitosan porous beads. The purified thrombin had a specific activity 1553.7U/mg so the purification activitg rate was 11.5 fold and recovery rate 78.8%.re spectively.

Key words: Thrombin, Chitosan porous beads, Affinity