Abstract: By using supercritical CO2 extraction, Litsea cubeba extraction oil is gotten. Bacteriostasis diameters to bacteria, yeast and molds were assayed with filter paper. And the influences to the bacteriostasis affect by using heating and changing the pH are also investigated. And then, the anti-oxidation effect of Litsea cubeba extraction oil is studied according to the peroxide value. The result showed that: since the bacteriostasis diameters of Litsea cubeba extraction oil on Bacillus subtilis and white Staphylococcus cremorcs are from 10～15mm, and the two bacteria are sensitive to the Litsea cubeba extraction oil. While, bacteriostasis diameters on Escherichia.coli, Aspergillusniger, Penicillium and Saccharmoyces cerevisiae are over 15mm, and the four bacteria are more sensitive to the Litsea cubeba extraction oil. the effect of Litsea cubeba extraction oil to the test organisms are great uner pH5.0, but get weaker with the increasing of pH. Treating the Litsea cubeba extraction oil in 80, 100, 121℃ for 15 mins, the bacteriostasis affect is not changed obviously. Mean while, the Litsea cubeba extraction oil has some kinds of anti-oxidation ability, but is weaker than TBHQ.

Key words: Litsea cubeba extraction oil, bacteriostasis, anti-oxidation, supercritical CO2 extraction