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Optimization of Supercritical CO2 Extraction Conditions of Polyphenols from Young Fruits of Holboellio latifolia by Response Surface Methodology

GU Renyong, YANG Wangen, YU Ji   

  1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China
  • Online:2015-05-25 Published:2015-05-08

Abstract:

Polyphenols were extracted from the young fruits of Holboellio latifolia by supercritical CO2 extraction. The
effect of ethanol concentration on extraction efficiency was examined. The extraction conditions solid/liquid ratio (namely
material/entrainer ratio), extraction pressure and temperature were investigated and the optimum extraction conditions were
obtained by response surface methodology. The results showed that the extraction yield and purity of polyphenols were
enhanced by 2.45 times and 15.49%, respectively, when using 95% ethanol as the entrainer, in comparison to those obtained
without the entrainer. The optimum extraction conditions for solid/liquid ratio, extraction pressure and temperature were 1:2.6
(g/mL), 37 MPa and 51 ℃, respectively. Under these conditions, the extraction yield and purity of polyphenols reached 6.41%
and 87.69%, respectively.

Key words: young fruits of Holboellio latifolia, polyphenols, supercritical CO2 extraction, entrainer, optimization by response surface methodology

CLC Number: