FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (1): 172-179.doi: 10.7506/spkx1002-6630-20200119-246

• Nutrition & Hygiene • Previous Articles     Next Articles

Curcumin Enhances the Antioxidant Capacity of Pheochromocytoma (PC12) Cells

XIAO Baoping, CHEN Lu, ZENG Jun, LIU Jingwen, LI Jian, LI Guiling,   

  1. (1. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; 2. Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China; 3. Engineering Technology Research Center for Marine Functional Food of Fujian Province, Xiamen 361021, China)
  • Published:2021-01-18

Abstract: Objective: Lowly and highly differentiated pheochromocytoma (PC12) cells were used to investigate the antioxidant capacity and underlying mechanism of curcumin. Methods: The relative proliferation rate of PC12 cells was measured by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, and the concentration and duration of curcumin treatment were determined. The effect of curcumin on the total antioxidant capacity (T-AOC) of PC12 cells was detected by 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) cation radical scavenging and ferric ion reducing antioxidant power (FRAP) methods. The level of reactive oxygen species (ROS) was measured by the dichloro-dihydro-fluorescein diacetate assay. The activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined using corresponding commercial kits, and the expression levels of antioxidant enzyme genes were detected by real-time fluorescent quantitative polymerase chain reaction. Results: Upon 10 μmol/L curcumin treatment for 24 h, the relative proliferation rate of lowly differentiated PC12 cells remained unchanged, while it was significantly inhibited in highly differentiated cells (P < 0.05). Treatment with curcumin at all concentrations tested significantly increased the T-AOC of both lowly and highly differentiated PC12 cells (P < 0.01), and reduced ROS level (P < 0.01). Furthermore, the activities and gene expression of SOD, CAT and GSH-Px were enhanced by curcumin. Conclusion: Curcumin can enhance the T-AOC, reduce ROS level, alleviate oxidative stress, and maintain cell homeostasis via increasing the activities and gene expression levels of SOD, CAT and GSH-Px.

Key words: plant polyphenols; pheochromocytoma cells; oxidative stress

CLC Number: