Isolation,Preparation and Identification of Hyperin from Acanthopanax Sessiliflorus (Rupr.et Maxim.) Seem fruit

Abstract

Abstract: Thin-layer chromatographic procedure was used to identify the composition of flavonoids of Acanthopanax Sessiliflorus(Rupr.et Maxim.)Seem purified by macroporous absorbing resins.The result showed that hyperin is one composition of the flavonoids.The preparative method of hyperin with HPLC and polyamide column were studied and the preparative hyperin was identified by UV,IR and NMR.The results showed that:the purity of the preparative hyperin can be up to 98%. UV,IR and NMR analysis proved that the furit hyperin is exploitable for further development.

Key words: Acanthopanax Sessiliflorus (Rupr.et Maxim) Seem, flavonoids, hyperin, preparation

FENG Ying, MENG Xian-Jun, WANG Jian-Guo. Isolation,Preparation and Identification of Hyperin from Acanthopanax Sessiliflorus (Rupr.et Maxim.) Seem fruit[J]. FOOD SCIENCE, 2007, 28(5): 298-300.

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Isolation,Preparation and Identification of Hyperin from Acanthopanax Sessiliflorus (Rupr.et Maxim.) Seem fruit

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