Abstract: This experiment studied the extraction, separation, purification and physico-chemical characteristics of acid proteoglycan extracted from Folium mori(APFM). The polysaccharides were extracted from F.mori by water extraction, and then isolated and purified by D101resin, DEAE-cellulose column and SephadexG-100 gel column chromatography successively. Monosaccharides composition analyses, acidic groups contents, and amino acids compositions were assayed with IR, UV, HPLC to for deduce the structural and physical and chemical properties. Results showed that APFM is a pure acidic proteoglycan withits molecular weight over 500 Da. It was constituted by D-rhamnose, L-arabinose, D-fructose, D-glucose and D-galactose with molar ratio: 8.91: 2.71: 1.00: 3.75: 6.04 respectively; and the contents of uronic acid, sulfate radical and protein 5.33%, 0.83% and 8.03% respectively. Its monomer of backbone is α-pyranose. In addition, APFM contains seventeen kinds of amino acids and seven kinds of which are human essential .

Key words:  , polysaccharides of F.mori； separation and purification； characterization；