Purification and Identification of Aspergillus ficuum Inulinases

Abstract

Abstract: Aspergillus ficuum inulinases were purified in a sequence of operations including ammonium sulfate fractionation, ion exchange chromatography on DEAE-Cellulose, column chromatography with Sepharose CL-6B and preparative electrophoresis. Five purified enzymes were thus obtained, two having an endo-type activity and the other being exo-actings. The five enzymes were identified as Exo-I, Exo-II, Exo-III, Endo-I and Endo-II, with MW(molecular weight) of 69961Da, 39973Da, 45561Da, 33574Da, 30769Da, respectively, determined by SDS-PAGE.

Key words: inulinase, purification, identification, Aspergillus ficuum

WANG Jing, JIN Zheng-Yu, JIANG Bo, XU Xue-Ming. Purification and Identification of Aspergillus ficuum Inulinases[J]. FOOD SCIENCE, 2007, 28(6): 188-192.

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Purification and Identification of Aspergillus ficuum Inulinases

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