Abstract

Abstract: A simple.rapid and accurate quantitative method was established for the determination of α-solanine inpotato by High-performance in potato were extracted with TFA-H2O-CH3CN-HACI50:30:20: 1,(V/V)]solution.Separation was obtained by using YWG C18 column (4.6mmi.d.x25mm,5 μ m) and mobile phase ofacctomitrile-0.02mol/L potassium dihydrogen phosphate (75:25.V/V) with fowl rate of 0.7ml/min.The eluates weremonitored with UV/VIS detector at 208nm.The retention time for α-solanine was 4.27min.The calibration curve waslinear in the range of 0.05～1.00mg/ml with r=0.9994.The average recovery tar α-solanine was 91 .5% with acoefficient of variation of 3 .84%.The content of α-solanine in potato from different areas was also determined.

Key words: , Potato &alpha, -solanine High-performance liquid chromatography；

WANG Shou-Lan, Zhu-Jia. [J]. FOOD SCIENCE, 1999, 20(6): 60-61.

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