Abstract: Extraction and purification of isoflavones from soybean powder were studied, and the effects of soybean isoflavones(10%purity) on senility of mice were analyzed too. The results showed that the isoflavones of 1.6% purity were obtained withthe conditions of solvent (60% enthanol) to soybean weight at 1:5 and 1 hour for immersion at 60℃, and the isoflavones of10% purity could be obtained by hydrolyzing the pre-extraction at acidity condition for 3 hours and separated throughmacropore resin columm. The results also showed that the skin hydroxyperoline contents of mice which been fed on isoflavonesfor 45 days increased at 0.05 level higher than the contrast, while the blood peroxide lipid contents of mice fed on isoflavonesfor 30 days decreased at 0.05 level lower.

Key words: Soybean , Isoflavone , Extraction , Purification , Anti-senility