Abstract: Based on the antibody-antigen-enzyme antibody reaction and by using puriftied Bt1 insecticidal crystal protein asboth standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was estab-lished to detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, Incomparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISAmethod might be useful for easy,rapid,reliable and effective assay in large samples.

Key words: Genetically modified maize , Bt1 protein , ELISA detection