FOOD SCIENCE ›› 1997, Vol. 18 ›› Issue (8): 7-10.

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The quick-screen typeof a glu-producing mutant with ICL biochemical genetic marker

 GAO  Huan-Chun,   吕Xiao-Ling,   Li-Wen-Ying   

  • Online:1997-08-15 Published:2011-12-19

Abstract:  The mutant Tbm-3with icl- (the isocitrate lyase deficieney) biochemical genetic marker was obtained directly from the parent strain T6-13, (Brevibacterium Tianjin) by selective breeding according to the quick--screen type (inoculated separately on two groups of three kinds planting culture medium) after mutagenesis using N-nitro-N’-methyl--N--nitroso--guanidine The L-Glu level accumulated and yield of L--Glu using Tbm--3was raise about 29 % separately against that using T6-13. This is a L-Glu--producing mutant with specific biochemical genetic markerby selective breeding using the quick--screen type that was designed according to metabolism theory of biochemistry. This method raised the efficiency of selective breeding greatly.

Key words:  , Quick-screen type; Mutation breeding; Isocitrate lyase; Biochernical defecitive mutant;