Abstract: Fermentation technology of Candida BH002, a 10-hydroxy-2-decenoic acid producing microorganism, was primarilystudied in this paper. Results showed that the optimum medium for the batch-fed fermentation was: sucrose 4%, ammoniumsulfate 0.3%, peptone 0.1%, beef extract 0.1%, dipotassium hydrogen phosphate 0.2%, royal jelly 0.5%, magnesium sulfate0.05%, ferrous sulfate 0.001% and CoCl2 0.001% and pH 7.0. The optimum initial pH value, dissoluble oxygen and reducingsugar concentration were 7.0, 25ml/.

Key words: hydroxy-2-decenoic acid Fermentation Biosynthesis