Quantitative Determination of Urease in Soybean by Ammonia Gas Sensing Electrode

Abstract

Abstract: Urease in soybean is determined quantitatively by ammonia gas sensing electrode based on the ammonia produced from the urease hydrolysis reaction of urea. The effects of temperature, pH and urea concentration on characteristic of urease in soybean is studied. The optimum temperature of soybean urease is 50℃. The pH value at which soybean urease shows supreme hydrolysis reaction is 8. 0. The suitable urea concentration is 0. 02 mol/ L. The linear range of steady-state response of ammonia gas sensing probe is 10 4-10-1 mol/L NH4Cl. This method has been applied to analysis of urease in soybean and gives recoveries of 97. 5% -103. 3% and standard deviation of 0. 55%-2. 65%.

Key words: , Ammonia Gas Sensing Electrode Urease；

Xu-Mao-Jun, LE Pei-恩, ZHENG Kai. Quantitative Determination of Urease in Soybean by Ammonia Gas Sensing Electrode[J]. FOOD SCIENCE, 1993, 14(9): 64-68.

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Quantitative Determination of Urease in Soybean by Ammonia Gas Sensing Electrode

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