Abstract: Trichoderma can widely contaminate mushroom and result in the decay of mushroom. In order to screen antifungal bacteria for the application in mushroom cultivation industry, 283 strains were isolated from 16 kinds of cultivation media. Of them, 16 exhibited obvious antifungal activity against Trichoderma, and 6 with the strongest antagonistic activity were screened out and the inhibitory effects of their fermentation supernatants were tested by the cup method. As a result, the best strain named as B155 was screened out with an inhibition zone radius of (19.02 ± 1.65) mm. According to morphological, physiological and biochemical characteristics as well as partial 16S rDNA sequence analysis, strain B155 was identified as B. licheniformis and designated as ABM 155. The optimal growth temperature, pH and inoculum amount for the strain were 37 ℃, 7.0 and 1%, respectively.

Key words: mushroom, Trichoderma, antagonistic bacteria, B. licheniformis, physiological characteristics