Screening of Trichoderma reesei Mutants and Optimization of Fermentation Conditions for Higher Cellulase Production

doi:10.7506/spkx1002-6630-20180403-032

Abstract

Abstract: Mutants of Trichoderma reesei RUT-C30 induced by atmospheric and room temperature plasma (ARTP) were screened for cellulase production. Whole-genome sequencing was performed to analyze the mutation types of the selected mutant. Also, we investigated the optimization of fermentation medium and conditions for improved production of cellulase. Mutant JNDY-13 was found to produce the highest cellulase activity. Through shake-flask fermentation, the filter paper activity (FPA) of JNDY-13 was 2.21 IU/mL, which was 2.21 times higher than that of T. reesei RUT-C30. The highest FPA of 5.40 IU/mL was achieved through fed-batch fermentation in a 5-L fermentor under optimized conditions. A total of 752 mutations were identified in JNDY-13, and the 18-base pair insertion in the galactokinase gene may account for the increased cellulase activity.

Key words: cellulase, Trichoderma reesei, whole-genome sequencing, mutation

CLC Number:

TQ939.97

ZOU Zongsheng, WANG Jingya, ZHAO Yunying, MAO Yin, DENG Yu. Screening of Trichoderma reesei Mutants and Optimization of Fermentation Conditions for Higher Cellulase Production[J]. FOOD SCIENCE, 2019, 40(6): 48-54.

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Screening of Trichoderma reesei Mutants and Optimization of Fermentation Conditions for Higher Cellulase Production

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