Abstract: In this investigation, the response surface methodology was employed to optimize the ionic liquid [BMIM]PF6- assisted enzymatic extraction of curcuma oil from turmeric powder. For this purpose, a regression model was developed to describe the yield of curcuma oil as a function of 4 independent variables including ionic liquid concentration, cellulase concentration, liquid-to-solid ratio and hydrolysis temperature. The optimum extraction parameters were determined as follows: ionic liquids concentration, 18% (V/V); cellulase concentration, 1.4% (m/m); liquid-to-solid ratio, 10:1 (mL/g); and hydrolysis temperature, 50 ℃. Under these conditions, the yield of curcuma oil was 5.0 mL/g. The essential oils obtained from SD and ILSE were analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 48 and 53 components were identified, respectively, which were mainly sesquiterpenes substance and its total relative content reached 74.49% and 84.57%, respectively. Compared with the steam distillation method, the volatile oil component of turmeric powder was increased. The volatile oil of turmeric had the same components, but their relative contents were different. The major components and their relative contents from SD and ILSE methods were ar-turmerone (42.86%, 39.37%), curlone (13.71%, 15.02%), and benzene,1-(1,5-dimethyl-4-hexen-1-yl)-4-methyl- (7.22%, 9.12%), respectively.

Key words: ionic liquid [BMIM]PF6, cellulase, curcuma oil, composition