Abstract: Experiments were conducted to study the effects of temperature,pH,protease and storage condition on the activity of native nisin through the inhibitory spectrum.The results indicated that nisin activity was affected significantly by temperature and pH.Under acidic condition nisin was stable to temperature.But as pH increased,the higher the temperature,the more the losses of nisin activity.The lower temperature was of advantage to nisin storage.Storage life of dry nisin was better than its solution.It was observed that native nisin was sensitive to trypsin,but insensitive to pepsin.The results of inhibitory spectrum experiments revealed that native nisin was inhibitive against Gram－ positive bacterium,but not inhibitive on Gram－ negative bacterium,yeast or mold.

Key words:  , Native nisin Characteristics；