Abstract: Tdoetermine Tempeh is oflavones inhibition on humabreast, uterus and ovary casncer cells. Thress cancer cells including MCF－7、Hela、Ho－8901 were treated by genistein、daidzein、glycitein、genistin、daidzin respectively, measuring the rate of inhibition and IC50. Genistein at 1.00E－04mol/L plrovided 63.08％rate of inhibition and IC50IC50=8.47E－05 in breast cancer MCF－7 cell、58.042％rate of inhibition and IC50=9.45E－05 in uterus cancer Hela cell、67.70％rate of inhibition and IC50=8.890E－05 in ovary cancer HO－8910 cell, respectively. Daidzein at 1.00E－04mol/L produced 67.70％control and IC50=2.62E－05 over ovary cancer HO－8910 cell.Tempeh isoflavone had higher antitumor activity than soybean isoflavone. Genistein showed has the higher control. Daindzein had effect only on ovary cancer cell. Genistin and Daidzintogethek showed no effect on three cancer cell.