SPE-HPLC Determination of 17α-Methyltestosterone Residues in Aquatic Products

Abstract

Abstract: A method to determine 17α-methyltestosterone (MET) residues in aquatic products was developed using LC-C18 solid phase extraction (SPE) combined with HPLC. Samples were extracted with diethyl ether under ultrasonic assistance. After concentration by rotary evaporation, the extract was defatted with n-hexane, cleaned up using an LC-C18 column, blown to dryness and dissolved again before reversed-phase HPLC analysis using a mobile phased made up of methanol and 0.1% formic acid (1:1, V/V). The average recovery rates of the HPLC method for MET at spike concentrations of 7.0–200 μg/kg were in the range of 82.0%–86.0% with a relative standard deviation (RSD) less than 7%. The limit of quantification (LOQ) for MET was 7.0 μg/kg.

Key words: 17α-methyltestosterone (MET), high performance liquid chromatography (HPLC), solid phase extraction (SPE), aquatic products

CLC Number:

TS207.3
O657

References

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