Detection and Homologous Analysis of CRISPR in Streptococcus thermophilus

FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (3): 153-157.

Detection and Homologous Analysis of CRISPR in Streptococcus thermophilus

Received:2011-10-28 Revised:2013-01-05 Online:2013-02-15 Published:2017-12-29
Contact: Kai-Bo DENG E-mail:keppel_red@yahoo.com.cn

Abstract

Abstract: CRISPR was recently found to be an acquired immune system widespread in prokaryotes. In order to find out CRISPRs in 8 strains of Streptococcus thermophilus isolated from Inner Mongolia, the CRISPR sequences were located by PCR amplification, and homology and secondary structure of direct repeat (DR) were predicted using bioinformatics method. Results showed that all the studied strains contained three CRISPR sequences except one in S4, which was much higher than the ratio (46.4%) in bacteria published on CRISPR database, and three CRISPRs had their specialized direct repeats (DR1-DR3)；The longest CRISPR was 2853 bp, and the shortest was only 101 bp. According to the prediction of secondary structure, a palindrome were detected in DR1-DR3, respectively, although the sizes of stem-loop were different; for the homology analysis, DR demonstrated high similarity with several individual species of distant relationships like those in the same genus or species, this suggested that the CRISPRs of tested strains also possess the characteristic of horizontal gene transfer (HGT), as well as the possibility of other evolutionary process.

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