Abstract: Objective: To compare the contents of flavonoids in tangerine peels of different varieties from Sichuan. Methods: The total flavonoids were determined by ultraviolet spectrophotometry at the wavelengths of 284 nm with hesperidin as a standard，and high performance liquid chromatography was used to analyze the contents of hesperidin, nobiletin and tangeretin simultaneously. The chromatographic separation was performed on a Phenomenex Luna C18(2) column (250 mm×4.6 mm，5 μm) by gradient elution using water-acetonitrile as the mobile phase at a flow rate of 1.0 mL?min-1. The detection wavelength was 280 nm and the column temperature was set at 25 ℃. Results: The results indicated that significan differences in contents of total flavonoids and three bioactive compounds existed in 20 samples from different source. The content ranges of total flavonoids，hesperidin, nobiletin, tangeretin were 0.64-5.67%，0.12-1.26%，0.02-0.61%，0.01-0.22%，respectively. The peak area of three flavonoids in samples had different proportion in total peak area. Conclusion: Flavonoids in tangerine peels were influenced by genetic background, growth environment as well as cultivation method. Multiple active chemical markers provided more quality information of tangerine peels.