FOOD SCIENCE ›› 0, Vol. ›› Issue (): 16-20.

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Separation, Purification and Structural Identification of Small Peptides from Jinhua Ham

  

  • Received:2011-05-09 Revised:2012-04-05 Online:2012-05-15 Published:2012-05-07

Abstract: Tangential flow filtration (TFF) and Sephadex G-15 gel filtration chromatography were used for the separation and purification of low molecular weight polypeptides in Jinhua ham at the end of sun-drying and post-ripening. Twenty-six main peptides were obtained and analyzed by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Seven of them had better repeatability and higher relative peak area. The amino acid sequences were Ala-His, Pro-Leu, Ser-Gly-Val, Ser-Gly-Phe, Pro-Leu-Lys-Pro-Ala, Leu-Leu-Ala-His and Asn-Pro-Thr, and the relative peak areas 17.81%, 8.62%, 10.58%, 5.70%, 6.11%, 6.12% and 3.49%, respectively.

Key words: jinhua ham, small peptide, separation and purification, LC-MS/MS, amino acid sequence

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