Molecular Phylogenic Identification of Wine-Related Yeasts

Abstract

Abstract: In this study, thirteen wine-related wild yeast strains from Shanshan, Xijiang were identified by PCR-RFLP (restriction fragment length polymorphism) and sequence analysis of the 5.8S-ITS region and the 26S rDNA D1/D2 domains. The PCR amplified 5.8S-ITS region were digested with four restriction endonucleases, Hae Ⅲ, Hin6Ⅰ, HinfⅠand AluⅠ to obtain 4 specific patterns. According to the sequence analysis of the 26S rDNA D1/D2 domains and BLAST alignment, the 13 yeast strains were identified as Saccharomyces cerevisiae, Candida zemplinina, Hanseniaspora uvarum and Pichia kluyveri var. kluyveri, while they were identified as Saccharomyces cerevisiae, Candida zemplinina, Hanseniaspora uvarum and Issatchenkia terricola according to the PCR-RFLP and sequence analysis of the 5.8S-ITS region. The same identification results for 10 of the 13 isolated yeast strains were obtained using the three methods, whereas inconsistent identification results were obtained for other three.

Key words: wine-related yeasts, molecular phylogeny, identification, 5.8S-ITS-RFLP, 26S rDNA D1/D2 sequence, 5.8S-ITS sequence

CLC Number:

Q93-331

References

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