Effects of Water-Soluble Organic Solvents on Activity of Chicken Liver Esterase

Abstract

Abstract: Chicken liver esterase, isolated from fresh chicken liver, is available for detecting pesticides. The effects of water-soluble organic solvents on the activity of chicken liver esterase were investigated. The results indicated that methanol, alcohol, propanol, ethylene glycol monomethyl ether (EGME) and glycol ether at low concentration could activate chicken liver esterase; in contrast, these organic solvents at high concentration could inactivate chicken liver esterase. Ethylene glycol monomethyl ether was the best organic solovent to maintain the activity of chicken liver esterase. When EGME concentration was 50%, the relative activity of chicken liver esterase remained 93%. Acetone and dioxane had negative effect on enzymatic activity. Acetone and dioxane at a concentration of 10% could result in 20% loss for relative activity of chicken liver esterase.

Key words: chicken liver esterase, enzymatic activity, organic solvents

CLC Number:

Jie ZHAO Fei Xu. Effects of Water-Soluble Organic Solvents on Activity of Chicken Liver Esterase[J]. FOOD SCIENCE, 2012, 33(15): 216-219.

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Effects of Water-Soluble Organic Solvents on Activity of Chicken Liver Esterase

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