Effects of Dietary Supplementation with Creatine Monohydrate and Conjugated Linoleic Acid on Muscle Fiber-Related Gene Expression and Enzyme Activities in Mice

doi:10.7506/spkx1002-6630-20200915-183

Abstract

Abstract: The aim of this study was to investigate the effects and underlying mechanisms of dietary supplementation with creatine monohydrate (CMH) and conjugated linoleic acid (CLA) on muscle fiber-related gene expression and enzyme activities in rats. Totally 40 healthy female Wistar rats were randomly divided into five groups coded A to E. Group A was fed a basic diet as the control group, group B the basic diet supplemented with 0.5% CMH, group C the basic diet supplemented with 1% CMH, group D the basic diet supplemented with 0.5% CLA , and group E the basic diet supplemented with 1% CLA. The feeding period lasted for four weeks. Compared to group A, group B showed a significant decrease in daily feed intake (P < 0.05), and a significant increase in the mRNA expression of myosin heavy chain IIa (MyHC IIa) and MyHC IIb, peroxisome-activated receptor coactivator 1α (PGC-1α), myocyte enhancer factor 2C (MEF2C), glucose transporter 4 (GLUT4), and succinate dehydrogenase (SDH) activity (P < 0.05); group C presented a significant decrease in daily feed intake (P < 0.05), and a significant increase in the mRNA expression of MyHC IIb, MEF2C and GLUT4 (P < 0.05). Compared to group A, group D showed a significant decrease daily feed intake and the mRNA expression of MyHC IIx (P < 0.05), and a significant increase in the mRNA expression of MyHC IIa and PGC-1α, and the activity of SDH, malate dehydrogenase, adenosine 5’-monophosphate-activated protein kinase (AMPK) (P < 0.05); group E exhibited a significant decrease in daily feed intake, lactic dehydrogenase (LDH) activity and the mRNA expression of MEF2C (P < 0.05), and a significant increase in the mRNA expression of MyHC I, MyHC IIa, PGC-1α and GLUT4, as well as SDH activity (P < 0.05). In summary, dietary supplementation with CMH or CLA activated AMPK in the muscle tissue of rats and increased the oxidative metabolism-related enzyme activities, up-regulated the mRNA expression of the muscle fiber-related genes PGC-1α, MEF2C and GLUT4, and enhanced the energy required for oxidative metabolism in muscle, thereby altering muscle fiber types.

Key words: creatine monohydrate; conjugated linoleic acid; muscle fiber type; enzymatic activity; muscle fiber type-related genes

CLC Number:

TS251.1

SU Lin, LI Huijiao, HOU Yanru, ZHAO Yajuan, BAI Yanping, SUN Bing, ZHAO Lihua, JIN Ye. Effects of Dietary Supplementation with Creatine Monohydrate and Conjugated Linoleic Acid on Muscle Fiber-Related Gene Expression and Enzyme Activities in Mice[J]. FOOD SCIENCE, 2021, 42(7): 149-155.

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Effects of Dietary Supplementation with Creatine Monohydrate and Conjugated Linoleic Acid on Muscle Fiber-Related Gene Expression and Enzyme Activities in Mice

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