Cloning and Molecular Characterization of f3′h Gene in Dendranthema morifolium

Abstract

Abstract: Dendranthema morifolium ‘chuju’, a traditional Chinese medicine resource unique to Chuzhou, Anhui, was used to clone flavonoid 3′-hydroxylase (f3′h) gene with a registration number of HQ256697 in NCBI by homology-based cloning strategy in this study. The results showed that a separate 381 bp DNA fragment was amplified by polymerase chain reaction (PCR) from three‘chuju’ samples collected from different places. Sequence analysis showed that the amplified DNA fragments were closely homologous to the 3rd exon of f3′h gene in Chrysanthemum morifolium (GU249553) at a homology rate of 98.69% for nucleotide sequences. Five SNPs in the homology area were observed, and only 3 of them resulted in amino acid sequence variations. The homology rates of nucleotide and amino acid sequences between Dendranthema morifolium‘chuju’and Sorghum bicolor, Brassica napus, Ipomoea nil Magenta, Malus domestica or Ipomoea purpurea were 60%～98% and 68%～97%, respectively. The classification of f3′h gene in different plants was significantly related to their genetic relationship. The f3′h gene of Dendranthema was more advanced than that ofother plants in system evolution.

Key words: Dendranthema morifolium‘chuju’, flavonoid, f3′h gene, the 3rd exon, sequence analysis

CLC Number:

Q949.9

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