Simultaneous Determination of 27 kinds of food additives and illegal additives in vegetable oil and seasoning oil by High-Performance Liquid Chromatography- Tandem Mass Spectrometry

Abstract

Abstract:

A high performance liquid chromatography- tandem mass spectrometry（HPLC- MS/MS）method for determination of 18 kinds of food additives and 9 kinds of illegal additives in vegetable oil and seasoning oil has been developed. Samples were diluted by hexane, extracted by acetonitrile and the acetonitrile layer was made up to constant volume before tested the 18 kinds of food additives. Hexane layer was concentrated before it cleansing through GPC, and the eluent was concentrated, made up to constant volume, at last the treated samples used HPLC-MS/MS assay. In the electrospray ion mode and MRM mode，27 kinds of compounds in the range show a good linear relationship, linear coefficients were greater than 0.983. The average recoveries ranged from 78.2% to 108.3%, the relative standard deviation is within0.9%-17.7%.This method can be used for testing the food additives and illegal additives in vegetable oil and seasoning oil. Applied this method in market samples detection，we found TBHQ in five kinds of samples，but，all of them was below the maximum limit in China.

CLC Number:

TS202.3

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