Optimization by Orthogonal Array Design of Sunflower Head Pectin Extraction and Characterization

doi:10.7506/spkx1002-6630-201314001

Abstract

Abstract:

The extraction of pectin from sunflower head was investigated. Different solvents were employed to extract
sunflower head pectin (SFHP). Major experimental conditions such as pH, temperature, extraction time, and solid-liquid
ratio were explored. Based on single-factor tests, the extraction parameters were further optimized using orthogonal tests.
Under the optimal extraction conditions: pH 3.5, 45 min, 85 ℃ and a solid-liquid ratio of 1:30 (g/mL), the yield of SFHP
in the form of galacturonic acid were up to 20.9%. High performance size exclusion chromatography-multi-angle laser
light scattering (HPSEC-MALLS) and high performance anion exchange chromatography-pulsed amperometric detection
(HPAEC-PAD) were used to determine the molecular weight and monosaccharide compositions of SFHP. The molecular
weight of SFHP was higher than that of citrus fruits and galacturonic acid was the major monosaccharide component of
SFHP, amounting for 82.1% of the total monosaccharides. Rhamnose was the second major monosaccharide, accounting for
only 9.1% of the total monosaccharides.

Key words: sunflower head, low methoxyl pectin, molecular mass, monosaccharide composition

CLC Number:

TS202.1

WANG Kun，HUA Xiao，YANG Rui-jin，KANG Jia-qi，ZHANG Wen-bin，ZHAO Wei. Optimization by Orthogonal Array Design of Sunflower Head Pectin Extraction and Characterization[J]. FOOD SCIENCE, 2013, 34(14): 1-5.

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