Optimizing an Ultrasound-Assisted Method for Extraction of Four Major Flavonoids in Barley by Using Orthogonal Regression Design

doi:10.7506/spkx1002-6630-201402010

Abstract

Abstract:

This study was attempted to develop the optimum ultrasound-assisted extraction (UAE) process of the four major
flavonoids catechin, myricetin, quercetin and kaempferol from barley using orthogonal array design and linear regression.
The contents of these flavonoid compounds in extracts were investigated with respect to methanol concentration, solid-tosolvent
ratio and sonication time. We proposed an optimized high performance liquid chromatography (HPLC) system to
determine the flavonoids. The analytical method displayed a good linear relationship for all the compounds tested in the
range of 0.005 - 0.1 μg, and was stable and reliable. The optimum extraction conditions were found using an extraction
solvent consisting of 50% methanol in water with a solid-to-solvent ratio of 1:20 (g/mL) and sonication for 60 min.

Key words: barley, HPLC, catechin, myricetin, quercetin, kaempferol

CLC Number:

YU Chun-lei, YUAN Jin-e, ZHANG Kun-fei, LUO Xiao-jiao, QI Guo-chang, FENG Zong-yun*. Optimizing an Ultrasound-Assisted Method for Extraction of Four Major Flavonoids in Barley by Using Orthogonal Regression Design[J]. FOOD SCIENCE, 2014, 35(2): 51-55.

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Optimizing an Ultrasound-Assisted Method for Extraction of Four Major Flavonoids in Barley by Using Orthogonal Regression Design

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