Abstract:

The optimal extraction conditions for flavonoids from Arenaria kansuensis Maxim. were determined using
response surface methodology (RSM). On the basis of single-factor tests, the effects of ethanol concentration, extraction
duration, extraction temperature, and liquid-material ratio on extraction rate were explored. A regression model describing
extraction rate of flavonoids as a function of three variables was established with the Design-Expert software according to
the Box-Behnken design principle and experimental data. Quadratic regression analysis was used to establish a mathematical
model describing flavonoids extraction. The optimal extraction conditions for flavonoids from Arenaria kansuensis Maxim.
were material-liquid ratio of 1:40 (g/mL), ethanol concentration of 68.6%, extraction temperature of 66 ℃ and extraction
time of 0.83 h. Under the optimal extraction conditions, the maximum extraction yield of flavonoids was 5.07 mg/g, which
was close to the predicted value of 5.10 mg/g. Therefore, the established regression model has good prediction capability.

Key words: Arenaria kansuensis Maxim., extraction, response surface methodology (RSM), flavonoids