Detection of Animal-Origin Ingredients in Edible Vegetable Oil by Real-Time PCR

doi:10.7506/spkx1002-6630-201314062

Abstract

Abstract:

In recent years, vegetable oil adulterated with animal-origin ingredients appeared in the markets. In this work, a
method for the detection of animal-origin ingredients in edible soybean oil by real-time fluorescence PCR was established,
and two primers for animal mitochondria 16S rRNA gene were designed on the basis of data reported in the literature.
For 5 samples of soybean oil adulterated with porcine, beef, sheep, chicken and fish oil, respectively, the animal-origin
mitochondria specific genes were sensitively detected with a detection limit of 0.05%.

Key words: edible vegetable oil, animal-origin ingredient, adulteration, real time polymerase chain reaction (PCR)

WANG Dan，ZHAO Lin-na，HU Feng-yue，WU Peng，HAN Yue-bei，YANG Hong-lian. Detection of Animal-Origin Ingredients in Edible Vegetable Oil by Real-Time PCR[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201314062.

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[12]	SHI Zihe, Josef VOGLMEIR, LIU Li. Recent Progress in Techniques for Adulteration Identification of Meat and Meat Products [J]. FOOD SCIENCE, 2019, 40(23): 319-326.
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Detection of Animal-Origin Ingredients in Edible Vegetable Oil by Real-Time PCR

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