Ultrasonic-assisted Enzymatic Extraction and Purification of Polysaccharides from Coprinus comatus Mycelia

doi:10.7506/spkx1002-6630-201316002

Abstract

Abstract:

Crude polysaccharides were obtained from Coprinus comatus mycelia using ultrasonication followed by cellulase
hydrolysis. The optimum extraction conditions that provided maximum extraction efficiency (3.48 ± 0.17) % were 500 W, 900
U/g (relative to the dry weight of Coprinus comatus mycelia), 2.5 h and 55 ℃ for microwave power, enzyme dose, extraction
time and temperature, respectively as determined by orthogonal array design. The crude polysaccharides were purified by
Sephadex G-200 and DEAE-32 column chromatography to obtain two major fractions. Both frations displayed a single
homogeneous band in cellulose acetate electrophoresis. Thus electrophoresis-pure polysaccharides were obtained.

Key words: Coprinus comatus mycelia, polysaccharides, purification, electrophoresis

CAO Xiang-yu，LIU Jian-li，QI Feng，HUO Ya-peng，TANG Xue-mei，LI Qi-jiu. Ultrasonic-assisted Enzymatic Extraction and Purification of Polysaccharides from Coprinus comatus Mycelia[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201316002.

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Ultrasonic-assisted Enzymatic Extraction and Purification of Polysaccharides from Coprinus comatus Mycelia

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