Identification, rDNA ITS Analysis and Carbon Metabolic Fingerprinting of a Pathogenic Fungus Isolated from Postharvest Garlic Sprout

doi:10.7506/spkx1002-6630-201315035

FOOD SCIENCE

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Identification, rDNA ITS Analysis and Carbon Metabolic Fingerprinting of a Pathogenic Fungus Isolated from Postharvest Garlic Sprout

WANG You-sheng，HE Xin-meng，ZHANG Yan，ZHANG Meng，LI Jian，LI Li-ping

Beijing Key Laboratory of Food Flavor Chemistry, Beijing Higher Institution Engineering Research Center of Food Additives and
Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China

Online:2013-08-15 Published:2013-09-03
Contact: WANG You-sheng

Abstract

Abstract:

A pathogenic fungus was isolated from infected garlic sprout during postharvest storage. Morphological
observation and rDNA ITS analysis indicated that the isolated strain was Stemphylium solani. Metabolic fingerprinting
investigation of 95 carbon sources suggested that the carbon metabolic fingerprinting of the strain was composed of 75
optimal carbon sources, including D-fructose, sucrose, maltose, α-D-glucose, D-galactose, and 5 available carbon sources
(D-glucosamine, D-tagatose, hydroxybutyric acid, D-malic acid and uridine) as well as 15 unavailable carbon sources such
as N-acetyl-D-galactosamine, N-acetyl-β-D-mannosamine and i-erythritol.

Key words: garlic sprout, postharvest disease, ITS rDNA sequence analysis, carbon metabolic fingerprinting

CLC Number:

Q93.331

WANG You-sheng，HE Xin-meng，ZHANG Yan，ZHANG Meng，LI Jian，LI Li-ping. Identification, rDNA ITS Analysis and Carbon Metabolic Fingerprinting of a Pathogenic Fungus Isolated from Postharvest Garlic Sprout[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201315035.

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Identification, rDNA ITS Analysis and Carbon Metabolic Fingerprinting of a Pathogenic Fungus Isolated from Postharvest Garlic Sprout

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