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Optimizing by Response Surface Methodology the Use of Microbial Transglutaminase to Improve Gelation Properties of Crocodylus siamensis Myofibril

LUO Zi-sheng,XIE Yan,LIU Ying-feng   

  1. School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
  • Online:2013-09-25 Published:2013-09-27

Abstract:

The optimal conditions of microbial transglutaminase (MTGase) for strengthening gelation properties of Crocodylus
siamensis myofibril were explored. The individual and interactive effects of incubation temperature, incubation time and MTGase
concentration on gel strength and water-holding capacity were investigated by one-factor-at-a-time design and response surface
analysis. The results showed that the optimal conditions for strengthening gelation properties of Crocodylus siamensis myofibril
were 38 ℃, 2.3 h and MTGase concentration of 0.8 U/g. Under the optimal conditions, the gel strength was 509.886 g•cm, and
water-holding capacity was 88.094%, which revealed an increase by 2.32-fold and 20.6% when compared with gels without
MTGase. These results demonstrated that the optimal hydrolysis procedure was feasible, and MTGase played an important role in
improving gelation properties of Crocodylus siamensis myofibril.

Key words: Crocodylus siamensis, myofibril, microbial transglutaminase (MTGase), gel strength, water-holding capacity, response surface methodology (RSM)