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Orthogonal Array Design for the Optimization of Enzymatic Hydrolysis and Spray Drying of Oyster Meat

WEI Hao-cheng,HE Chuan-bo,TANG Feng-xia,WU Guo-hong,XIONG He-jian   

  1. Fujian Province Key Laboratory of Food Microorganism and Enzyme Engineering, College of Biological Engineering,Jimei University, Xiamen 361021, China
  • Online:2013-09-25 Published:2013-09-27

Abstract:

Fresh oysters were hydrolyzed by a commercial mixture of acid proteases, deodorized and spray-dried to produce
oyster powder. The three procedures were investigated. The optimum conditions for enzymatic hydrolysis of oyster that
provided maximum amino acid nitrogen content were found to be 3 h of hydrolysis at 60 ℃ with an enzyme dosage of 0.15%
as determined by orthogonal array design. The optimum conditions for deodorizing the hydrolysate obtained were 1 h of
incubation at 37 ℃ after the addition of 1% yeast extract as determined by sensory evaluation, and spray-dried powder of the
hydrolysate under the conditions: addition of 10% maltodextrin, air inlet temperature 220 ℃ and peristaltic pump speed 200
mL/h exhibited the best sensory quality.

Key words: oyster powder, enzymatic hydrolysis, deodorization, spray drying