Abstract:

A method to detect phloridzin in apple buds was established using high performance liquid chromatography
(HPLC) with a diode array detector (DAD). Chromatographic separation was conducted on a Diamonsil C18 (250 mm ×
4.6 mm, particle size 5 μm) with a gradient elution program using a mobile phase made up of 1% acetic acid (V/V) and
methanol as at 280 nm. The method had a linear range from 6.25 to 100 μg/mL with correlation coefficient of 0.9999, the
limit of detection was 0.0313 μg/mL and the limit of quantification was 0.0939 μg/mL. The recovery ranged from 95.38%
to 100.12%. Phloridzin in apple buds from 10 different varieties (Fuji, Ralls, Jonagold, Pink lady, Qinyang, Qinguan, Gala,
Granny Smith, Jonathan, Starkrimzon) grown in Baishui county, Shaanxi province were determined by the HPLC method.
The results showed that the highest content of phloridzin in Starkrimzon buds was 17.284 mg/g among the varieties studied,
and the lowest content in Qinyang was 12.569 mg/g.

Key words: apple buds, phloridzin, high performance liquid chromatography (HPLC)