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Preparation of High Purity Capsaicin Monomer by Reverse Phase C18 Column Chromatography

WANG Yang，HAN Shuang，CAO Di，LIU Ju，ZHOU Yun-peng，XU Li-feng，CHEN Ye   

  1. 1. College of Pharmacy, Liaoning University, Shenyang 110036, China；
    2. Liaoning Cancer Hospital & Institute, Shenyang 110042, China
  • Online:2013-12-25 Published:2014-01-03

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Abstract:

A novel method was developed to prepare high purity capsaicin monomer using reverse phase C18 column
chromatography. Capsaicinoids were extracted from capsicum oleoresin and then fractionated by column chromatographies
on silica gel, alumina and reverse phase C18 silica gel. As a result, a mixture was obtained consisting of capsaicin with
a purity of 98% as determined by HPLC and dihydrocapsaicin. This study suggests that reverse phase C18 column
chromatography is an effective method to purify capsaicinoids and prepare high-purity capsaicin monomer.

Key words: capsaicin monomer, capsaicinoids, reverse phase C18 column chromatography