Abstract:

Purpose: To study the inhibitory effect of rutin on the formation of advanced glycation end products (AGEs)
in bovine serum albumin (BSA) system caused by sugar or dicarbonyl compounds metabolized by sugar. Methods: BSAMGO
(methylglyoxal), BSA-GO (glyoxal) and BSA-reducing sugar reaction systems were established. The effects of MGO,
GO and reducing sugar concentrations on the formation of AGEs were investigated by fluorescence spectrophotometry.
The inhibitory effect of rutin on the formation of AGEs was investigated at various concentrations added in three reaction
systems. Results: Non-enzymatic glycation of BSA was obviously observed in these three systems with MGO, GO and sugar
at 0.5, 0.5 mmol/L and 10 mmol/L, respectively. Rutin addition at concentrations of 0.25, 0.25 mmol/L and 1 mmol/L could
effectively inhibit the formation of AGEs in the three systems. Conclusion: Rutin can effectively inhibit AGEs in BSAMGO,
BSA-GO and BSA-sugar systems.

Key words: bovine serum albumin, dicarbonyl compounds, rutin, advanced glycation end product