Abstract: The objective of this work was to explore the inhibitory effect of oligomeric procyanidins of lotus seedpod (LSOPC) on the formation of advanced?glycation?end?products?(AGEs) in a simulated physiological environment. The percentage inhibition of AGEs by LSOPC in the presence of metal ions, high glucose and high protein concentration was determined and the inhibitory effect of LSOPC complexes with epigallocatechin gallate (EGCG) on the formation of AGEs was evaluated. The?results?showed?that?the?optimal?incubation time to obtain the highest inhibitory effect of LSOPC was 35 d. Inhibitory effects on AGEs formation varied depending on the type and concentration of metal irons added. Cu2+ and Fe2+ had the highest promoting effects on AGEs formation and could work together with LSOPC. Five other metal irons had different inhibitory or promoting effects on AGEs formation but hardly influenced the effect of LSOPC. We also found that LSOPC had the lowest inhibitory effect at high concentrations of both BSA and glucose or at high concentrations of BSA. Additionally, LSOPC in combination with EGCG showed a potent synergistic inhibitory effect and the optimal effect was obtained at a ratio of 2:1.

Key words: oligomeric procyanidins of lotus seedpod (LSOPC), advanced glycation end products (AGEs), simulate physiological environment, inhibitory effect