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Active Bacterial Community Diversity in Traditional Fermented Soybean in Yunnan Using Mixed Culture and Barcoded Pyrosequencing

LI Xiao-ran, GONG Fu-ming, LI Jie, LUO Yi-yong, LIU Chen-jian   

  1. 1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China;
    2. Basic Department, Dehong Vocational College, Mangshi 678400, China
  • Online:2014-04-15 Published:2014-04-18

Abstract:

In this study, bacterial communities in two samples of traditional fermented soybean in Yunnan province wereanalyzed by the combined use of mixed culture and 16S rRNA gene barcoded pyrosequencing. Results revealed highbacterial community diversities of both samples at a 0.02 cutoff. In sample XG_1, the major bacteria were Enterococcusspp., Bacillus methylotrophicus and Pediococcus acidilactici. In sample XG_2, the abundant bacteria were Bacillus subtilisand B. methylotrophicus.

Key words: fermented soybean, mixed culture, bacterial community, pyrosequencing