Optimization of Fermentation Conditions for the Production of Exopolysaccharides by Lactobacillus helveticus MB2-1

doi:10.7506/spkx1002-6630-201407020

Abstract

Abstract:

The fermentation conditions for the production of exopolysaccharides (EPS) by Lactobacillus helveticusMB2-1 isolated from Sayram traditional yogurt in Xinjiang were optimized. The effects of culture temperature, lactoseconcentration, nitrogen source and concentration, and mineral source and concentration on EPS yield were studied throughsingle factor analysis. Response surface analysis (RSM) involving three independent variables at three different levels wascarried out to determine the optimal process conditions by using Box-Behnken method. Results showed that the optimumextraction conditions were 37 ℃, 0.2 g/100 mL and 2 g/100 mL for culture temperature, Mg2+ concentration and soybean peptoneconcentration, respectively. Under these conditions, the yield of EPS by L. helveticus MB2-1 was as high as 801.2 mg/L.

Key words: Lactobacillus helveticus MB2-1, exopolysaccharides (EPS), fermentation, response surface methodology (RSM)

JI Juan, LI Wei, CHEN Xiao-hong, JIANG Mei, RUI Xin, DONG Ming-sheng. Optimization of Fermentation Conditions for the Production of Exopolysaccharides by Lactobacillus helveticus MB2-1[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201407020.

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Optimization of Fermentation Conditions for the Production of Exopolysaccharides by Lactobacillus helveticus MB2-1

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